Year : 2004  |  Volume : 11  |  Issue : 2  |  Page : 93-102

Quinalphos induced oxidative stress and histoarchitectural alteration in rat testis

Animal Based Physiology Research Laboratory, Biophysics Division, Saha Institute of Nuclear Physics, 37, Belgachia Road, Kolkata -700 037, India

Correspondence Address:
T K Mandal
Animal Based Physiology Research Laboratory, Biophysics Division, Saha Institute of Nuclear Physics, 37, Belgachia Road, Kolkata -700 037
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The effect of quinalphos at two sublethal different doses - one at low (QP-250μg / kg b. wt.) and other at high dose (QP-500μg /kg. b .wt) for variable durations (3, 8 and 15 days respectively) on oxidative stress and testicular responsiveness as revealed by the histopathological and biochemical changes in albino adult rats, were studied. At low dose QP treatments for 3, 8 and 15 days, respectively, the changes in testicular activities in relation to testicular oxidative stress were pronounced and as a result, shrinkage of tubular diameter and testicular atrophy leading to the degenerative changes in the germinal epithelium, detrimental changes in various layers of the seminiferous tubular cells with overall significant reduction in the number of germ cell at stage VII spermatogenesis cell cycles were found. Quantitative studies of spermatogenesis revealed that a significant reduction in the number of type A spermatocytes (mPSC) and stage 7 spermatids (7Sd) at stage VII of the seminiferous epithelium cell cycles was observed at low dose QP treatment as compared with the number in corresponding vehicle (control groups) treated controls. Maximum decrease in number of 7Sd was observed from the groups treated with lower dose. Similar effects were also observed in the number of sertoli cells in the tubules after the treatment. But in the high dose treatments gradual recovery effects were noticed with significant expansion of seminiferous tubules and restoration of spermatogenesis to the control level. Similar changes in biochemical profiles such as the products of free radicals like manoldialdehyde (MDA)/lipid peroxides as well as the activities of testicular antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), Glutathione dismutase peroxide (GSH-PX), lipid and protein profiles etc. were also observed. After the treatment with low dose of quinalphos, there was an increase in the lipid peroxides or thiobarbituric acid reaction substances (TBARS)/MDA (as measured by manoldialdehyde) and a decrease in the total lipid content for the testicular membrane. Quinalphos induced oxidative stress and decreased antioxidative and antiperoxidative enzymes systems at low dose of QP resulting in the drastic changes in testicular morphology and cell architecture. On the other hand, high dose QP treatment caused lesser but gradual recovery effect. These later restoration effects may presumably be regarded as recovery effect possibly through available FSH and testosterone.

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