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| Year : 2004 | Volume
: 11
| Issue : 2 | Page : 85-91 |
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Toxicity of respirable ambient particulates from different locations of an urban dwelling : An in vitro cytotoxicity study in alveolar macrophages of rats
A Pradhan1, M Waseem1, S Dogra1, AK Khanna1, SN Khattri2, JL Kaw1
1 Pulmonary Toxicology Division, Industrial Toxicology Research Centre, M.G. Marg, P.O. Box 80, Lucknow, India
2 Department of Zoology, DBS Postgraduate College, Kanpur (U.P.), India
Correspondence Address:
M Waseem
Pulmonary Toxicology Division, Industrial Toxicology Research Centre, M.G. Marg, P.O. Box 80, Lucknow
India
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In the present study, potential of sampled respirable suspended particulate matter (RSPM) to produce in vitro cytotoxicity to rat alveolar macrophages was investigated and studied with reference to samples of different locations of collection, duration of exposure, quantum of exposure and metal contents. the samples were collected from selected points covering residential, commercial and industrial areas of an urban settling. The study indicated that the exposure to RSPM decreased the cell viability to a significant extent. the release of cytoplasmic lactate dehydrogenase (LDH) in the supematant was found significantly enhanced. Nitric oxide (NO), a pro-inflammatory response molecule, was enhanced in short exposure, where as on prolonged exposures the percentage comparison was reduced with respect to controls. We concluded that RSPM of different locations on exposure to pulmonary alveolar macrophages manifested different response, which was a sum total or individual interaction of all physical, chemical and biological components. The reaction of RSPM in the exposure environment played a key role in imparting the degree of cytotoxicity |
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