ARTICLE
Year : 2004  |  Volume : 11  |  Issue : 2  |  Page : 107-113

Effect of Ocimum tenuiflorum extracts on MDBK cell proliferation in vitro


1 Division of Biochemistry and Food Science, Izatnagar-243122, UP, India
2 Chembioteck International Pvt. Ltd, Kolkatta, India
3 National Biotechnology Centre, Izatnagar-243122, UP, India

Correspondence Address:
M Saini
Division of Biochemistry and Food Science, Izatnagar-243122, UP
India
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The effect of seed and leaf extracts of Ocimum tenuiflorum on MDBK cell proliferation was studied in vitro. MDBK cells (5x105/100 μl) were incubated with serial two fold dilutions of each extracts in quadruplicate well of a 96 well tissue culture plate. The plates were incubated at 37C in a humidified CO2 incubator for 48 h. The effect on the cell proliferation was measured by the extent of cell toxicity caused by the measured by lactate dehydrogenase (LDH) activity in the cell culture supernatant and concomitant decrease in the cell viability as quantified by mitochondrial dehydrogenase activity by MTT reduction assay in the cell monolayer. Aqueous and alcoholic extract of dried leaves, aqueous and boiled aqueous extract of fresh leaves, decoction of fresh leaves and aqueous extract of seeds were found to cause toxicity at higher concentration as evidenced by decreased MTT reduction and increased activity of LDH in the treated cells as compared to the control cells. The MDBK cell monolayer treated with the highest concentration of the extracts for 4 at 37C demonstrated some cell death that was associated with decrease in cellular glutathione, decreased activities of glutathione-S-transferase and glucose-6-phosphate dehydrogenase, indicating oxidative stress. However, the lower concentration of the extracts was found to boost the cell proliferation. The factor responsible appeared to be polar and heat stable in nature. This in vitro model of toxicity testing can be used for screening cytotoxic concentrations of drugs and chemicals.


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